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BOD Bottle BOD test bottles at the laboratory of a wastewater treatment plant. BOD Bottle or an incubation bottle is a main apparatus used for the Biological Oxygen Demand (BOD) test. During the five-day BOD or BOD5 test process, the BOD bottle is used for incubating diluted samples under the 20 °C or 68 °F of temperature.
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Common cap sizes include 33-430 (33mm), 38-430 (38mm), and GL 45 (45mm). Caps range in size from narrow mouthed to wide mouthed and often a glass or plastic funnel is needed to properly fill a reagent bottle from a larger or equal sized container's mouth. Reagent bottle caps are commonly said to be "autoclavable".
24/40 is common for 250 mL or larger flasks, while smaller sizes such as 14/20 or 19/22 are used for smaller flasks. Because of the round bottom, cork rings are needed to keep the round bottom flasks upright. When in use, round-bottom flasks are commonly held at the neck by clamps on a stand.
The BOD test is not bottle dependent, many labs use a 500ml bottle for the samples under test if using a titration method for determination or as small as 50ml if using an electronic DO probe, but the size of the bottle is only a function of practicality and space.
For instance, a 250 mL beaker might be marked with lines to indicate 50, 100, 150, 200, and 250 mL of volume. These marks are not intended for obtaining a precise measurement of volume (a graduated cylinder or a volumetric flask would be a more appropriate instrument for such a task), but rather an estimation. Most beakers are accurate to ...