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Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]
In single-end sequencing, there is only one read per fragment (i.e., RPM = FPM). In paired-end sequencing, there are two reads per fragment ( i.e. , RPM = 2 x FPM). Sequencing depth is sometimes referred to as library size , the number of intermediary cDNA molecules in the experiment.
In genetics, the gene density of an organism's genome is the ratio of the number of genes per number of base pairs, usually written in terms of a million base pairs, or megabase (Mb). The human genome has a gene density of 11-15 genes/Mb, while the genome of the C. elegans roundworm is estimated to have 200.
MGISEQ-2000: 375M FCS flow cell, 1500M FCL flow cell per flow cell. 1 to 9 days depending on instrument, read length and number of flow cells run at a time. $5– $120 Sequencing by ligation (SOLiD sequencing) 50+35 or 50+50 bp: 99.9%: 1.2 to 1.4 billion: 1 to 2 weeks: $60–130: Low cost per base. Slower than other methods.
[1] [2] It is usually given in units of becquerel per kilogram (Bq/kg), but another commonly used unit of specific activity is the curie per gram (Ci/g). In the context of radioactivity , activity or total activity (symbol A ) is a physical quantity defined as the number of radioactive transformations per second that occur in a particular ...
The specific weight, also known as the unit weight (symbol γ, the Greek letter gamma), is a volume-specific quantity defined as the weight W divided by the volume V of a material: = / Equivalently, it may also be formulated as the product of density, ρ, and gravity acceleration, g: = Its unit of measurement in the International System of Units (SI) is newton per cubic metre (N/m 3), with ...
Genome size ranges (in base pairs) of various life forms. Genome size is the total amount of DNA contained within one copy of a single complete genome.It is typically measured in terms of mass in picograms (trillionths or 10 −12 of a gram, abbreviated pg) or less frequently in daltons, or as the total number of nucleotide base pairs, usually in megabases (millions of base pairs, abbreviated ...
Although there are examples to the contrary, it should be stressed that the commonly used "units" of % w/v are grams per millilitre (g/mL). 1% m/v solutions are sometimes thought of as being gram/100 mL but this detracts from the fact that % m/v is g/mL; 1 g of water has a volume of approximately 1 mL (at standard temperature and pressure) and ...