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A monolithic HPLC column, or monolithic column, is a column used in high-performance liquid chromatography (HPLC). The internal structure of the monolithic column is created in such a way that many channels form inside the column. The material inside the column which separates the channels can be porous and functionalized.
HPLC has many applications in both laboratory and clinical science. It is a common technique used in pharmaceutical development, as it is a dependable way to obtain and ensure product purity. [59] While HPLC can produce extremely high quality (pure) products, it is not always the primary method used in the production of bulk drug materials. [60]
Chromatography columns of different types are used in both gas and liquid chromatography: Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer ...
Proper column packing is important for maximum resolution: An over-packed column can collapse the pores in the beads, resulting in a loss of resolution. An under-packed column can reduce the relative surface area of the stationary phase accessible to smaller species, resulting in those species spending less time trapped in pores.
In HPLC, the capacity factor represents the molar ratio of the solute in the stationary phase to the mobile phase. The capacity factor is easily measure based on retention times of the compound and any unretained compound. The equation rewritten by Guermouche et al. [9] is presented here: 1/k¢ = [n • (KMW-1)/(f • KSW)] • CM +1/(f • KSW ...
Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) [1] is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography.
The column is the most important component of the LC system and is designed to withstand the high pressure of the liquid. Conventional LC columns are 100–300 mm long with outer diameter of 6.4 mm (1/4 inch) and internal diameter of 3.0–4.6 mm. For applications involving LC–MS, the length of chromatography columns can be shorter (30–50 ...
Chromatography, pronounced / ˌ k r oʊ m ə ˈ t ɒ ɡ r ə f i /, is derived from Greek χρῶμα chrōma, which means "color", and γράφειν gráphein, which means "to write".". The combination of these two terms was directly inherited from the invention of the technique first used to separate biological pigme
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