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The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a suitable bacterial strain , but other organisms such as yeast may also be used.
An alternative to a selectable marker is a screenable marker, another type of reporter gene which allows the researcher to distinguish between wanted and unwanted cells or colonies, such as between blue and white colonies in blue–white screening. These wanted or unwanted cells are simply non-transformed cells that were unable to take up the ...
Blue white screen is used in both bacteria and eukaryotic cells. The bacterial lacZ gene encodes a beta-galactosidase enzyme. When media containing certain galactosides (e.g. X-gal ), cells expressing the enzyme convert the X-gal to a blue product and can be seen with the naked eye.
Such features present in cloning vectors may be the lacZα fragment for α complementation in blue-white selection, and/or marker gene or reporter genes in frame with and flanking the MCS to facilitate the production of fusion proteins. Examples of fusion partners that may be used for screening are the green fluorescent protein (GFP) and ...
Anthony Mackie taking on the role of Captain America, a fictional comic book character initially portrayed as a white man with blond hair and blue eyes, isn’t groundbreaking to comic books fans.
This allows for blue–white screening when using host strains such as E. coli JM109, which produces only the C-terminal portion of lacZ, also known as the β-polypeptide. [3] If pUC19 is inserted into E. coli JM109 and grown on agar media supplemented with IPTG and X-gal , then colonies will appear blue, as the plasmid encodes for the α ...
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DH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three [1] mutations: recA1, endA1 which help plasmid insertion and lacZΔM15 which enables blue white screening.