Search results
Results from the WOW.Com Content Network
The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a suitable bacterial strain , but other organisms such as yeast may also be used.
An alternative to a selectable marker is a screenable marker, another type of reporter gene which allows the researcher to distinguish between wanted and unwanted cells or colonies, such as between blue and white colonies in blue–white screening. These wanted or unwanted cells are simply non-transformed cells that were unable to take up the ...
Such features present in cloning vectors may be the lacZα fragment for α complementation in blue-white selection, and/or marker gene or reporter genes in frame with and flanking the MCS to facilitate the production of fusion proteins. Examples of fusion partners that may be used for screening are the green fluorescent protein (GFP) and ...
This allows for blue–white screening when using host strains such as E. coli JM109, which produces only the C-terminal portion of lacZ, also known as the β-polypeptide. [3] If pUC19 is inserted into E. coli JM109 and grown on agar media supplemented with IPTG and X-gal , then colonies will appear blue, as the plasmid encodes for the α ...
DH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three [1] mutations: recA1, endA1 which help plasmid insertion and lacZΔM15 which enables blue white screening.
The Ishihara test is a color vision test for detection of red–green color deficiencies.It was named after its designer, Shinobu Ishihara, a professor at the University of Tokyo, who first published his tests in 1917.
Testing the colors of a web chart, (center), to ensure that no information is lost to the various forms of color blindness. Color codes are useful tools for designers to convey information. The interpretation of this information requires users to perform a variety of color tasks, usually comparative but also sometimes connotative or denotative.
An Ishihara test image as seen by subjects with normal color vision and by those with a variety of color deficiencies. A pseudoisochromatic plate (from Greek pseudo, meaning "false", iso, meaning "same" and chromo, meaning "color"), often abbreviated as PIP, is a style of standard exemplified by the Ishihara test, generally used for screening of color vision defects.