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A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10 0.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10 0.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution.
For example, on a plot axis showing log 2 fold changes, an 8-fold increase will be displayed at an axis value of 3 (since 2 3 = 8). However, there is no mathematical reason to only use logarithm to base 2, and due to many discrepancies in describing the log 2 fold changes in gene/protein expression, a new term "loget" has been proposed. [10]
Fluorescein aqueous solutions, diluted from 10,000 to 1 parts-per-million in intervals of 10 fold dilution. At 1 ppm the solution is a very pale yellow. At 1 ppm the solution is a very pale yellow. As the concentration increases the colour becomes a more vibrant yellow, then orange, with the final 10,000 ppm a deep red colour.
Therefore, to ensure that a sample will yield CFU in this range requires dilution of the sample and plating of several dilutions. Typically, ten-fold dilutions are used, and the dilution series is plated in replicates of 2 or 3 over the chosen range of dilutions. Often 100 μL are plated but also larger amounts up to 1 mL are used.
Owners push a button, and the rooms fold out like an accordion using a counterbalance system. The company claims that once a home folds out, its parts only need to be secured with an electric drill.
The "dilution factor" is an expression which describes the ratio of the aliquot volume to the final volume. Dilution factor is a notation often used in commercial assays.
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