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A prominent example is the bacterial mesosome, which was thought to be an organelle in gram-positive bacteria in the 1970s, but was later shown by new techniques developed for electron microscopy to be simply an artifact of chemical fixation. [2] [3] Standardization of fixation and other tissue processing procedures takes this introduction of ...
Cryofixation is a technique for fixation or stabilisation of biological materials as the first step in specimen preparation for the electron microscopy and cryo-electron microscopy. [1] Typical specimens for cryofixation include small samples of plant or animal tissue , cell suspensions of microorganisms or cultured cells , suspensions of ...
Paraformaldehyde can be depolymerized to formaldehyde gas by dry heating [2] and to formaldehyde solution by water in the presence of a base, an acid or heat. The high purity formaldehyde solutions obtained in this way are used as a fixative for microscopy and histology. The resulting formaldehyde gas from dry heating paraformaldehyde is flammable.
Typically, embalming fluid contains a mixture of formaldehyde, glutaraldehyde, methanol, and other solvents. The formaldehyde content generally ranges from 5–37% and the methanol content may range from 9–56%. In the United States alone, about 20 million liters (roughly 5.3 million gallons) of embalming fluid are used every year. [1]
This page contains tables of azeotrope data for various binary and ternary mixtures of solvents. The data include the composition of a mixture by weight (in binary azeotropes, when only one fraction is given, it is the fraction of the second component), the boiling point (b.p.) of a component, the boiling point of a mixture, and the specific gravity of the mixture.
The stock solution for Karnovsky fixative is as follows: [1] 2.0 g paraformaldehyde; 25 ml distilled water; 1M sodium hydroxide 2 to 4 drops; 50% glutaraldehyde 5.0 ml; 0.2M cacodylate buffer, pH 7.4, 20.0 ml; Mix the paraformaldehyde with 25 ml of distilled water in a 125 ml Erlenmeyer flask. Heat to 60 °C on a stir plate.
This Wikipedia page provides a comprehensive list of boiling and freezing points for various solvents.
The compound is closely related and convertible to the industrially significant derivatives paraformaldehyde ((CH 2 O) n), formaldehyde (H 2 C=O), and 1,3,5-trioxane ((CH 2 O) 3). [3] Methanediol is a product of the hydration of formaldehyde. The equilibrium constant for hydration is estimated to be 10 3, [4] CH 2 (OH) 2 predominates in dilute ...