Search results
Results from the WOW.Com Content Network
Some enzymes known as "exo-endonucleases", however, are not limited to either nuclease function, displaying qualities that are both endo- and exo-like. [1] Evidence suggests that endonuclease activity experiences a lag compared to exonuclease activity.
Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end (exo) of a polynucleotide chain. A hydrolyzing reaction that breaks phosphodiester bonds at either the 3′ or the 5′ end occurs. Its close relative is the endonuclease, which cleaves phosphodiester bonds in the middle (endo) of a polynucleotide chain.
In biochemistry, a nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds that link nucleotides together to form nucleic acids. Nucleases variously affect single and double stranded breaks in their target molecules.
Exonuclease III (ExoIII) is an enzyme that belongs to the exonuclease family. ExoIII catalyzes the stepwise removal of mononucleotides from 3´-hydroxyl termini of double-stranded DNA. [1]
Reaction diagrams for both hydrolytic (left) and phosphorolytic (right) 3'-5' exoribonuclease degradation of RNA. An exoribonuclease is an exonuclease ribonuclease, which are enzymes that degrade RNA by removing terminal nucleotides from either the 5' end or the 3' end of the RNA molecule.
Micrococcal nuclease (EC 3.1.31.1, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T', staphylococcal nuclease, spleen phosphodiesterase, Staphylococcus aureus nuclease, Staphylococcus aureus nuclease B, ribonucleate (deoxynucleate) 3'-nucleotidohydrolase) is an endo-exonuclease that preferentially digests single-stranded nucleic acids.
Then, MNase-seq uses the endo-exonuclease micrococcal nuclease to bind and cleave protein-unbound regions of DNA of eukaryotic chromatin, first cleaving and resecting one strand, then cleaving the antiparallel strand as well. [3] The chromatin can be optionally crosslinked with formaldehyde. [44]
24127 Ensembl ENSG00000114127 ENSMUSG00000032410 UniProt Q8IZH2 P97789 RefSeq (mRNA) NM_001042604 NM_001282857 NM_001282859 NM_019001 NM_011916 NM_001311130 RefSeq (protein) NP_001269786 NP_001269788 NP_061874 n/a Location (UCSC) Chr 3: 142.31 – 142.45 Mb Chr 9: 95.84 – 95.94 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse 5′-3′ exoribonuclease 1 (Xrn1) is a protein that in ...