Search results
Results from the WOW.Com Content Network
Western blot workflow. The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1]
A western blot is used for the detection of specific proteins in complex samples. Proteins are first separated by size using electrophoresis before being transferred to an appropriate blotting matrix (usually polyvinylidene fluoride or nitrocellulose ) and subsequent detection with antibodies.
Immunoprecipitation of intact protein complexes (i.e. antigen along with any proteins or ligands that are bound to it) is known as co-immunoprecipitation (Co-IP). Co-IP works by selecting an antibody that targets a known protein that is believed to be a member of a larger complex of proteins.
What links here; Related changes; Upload file; Special pages; Permanent link; Page information; Cite this page; Get shortened URL; Download QR code
The southwestern blot, is a lab technique that involves identifying as well as characterizing DNA-binding proteins [1] by their ability to bind to specific oligonucleotide probes. Determination of molecular weight of proteins binding to DNA is also made possible by the technique.
Polysorbate 20 is also known as Tween 20, a commercial brand name. It is a common detergent used in many buffers for washing nitrocellulose membrane in western blotting and microtiter plate wells in ELISA assays. Tris is a buffer that maintains a pH of 7–9.2.
A review on western blotting from 2006 describes Towbin as the author for W.blotting and Stark for Northern -- maxhebditch 14:10, 07/01/13 —Preceding undated comment added 14:12, 7 January 2013 (UTC) [ reply ]
Normalization of Western blot data is an analytical step that is performed to compare the relative abundance of a specific protein across the lanes of a blot or gel under diverse experimental treatments, or across tissues or developmental stages.