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Generally, a pipette has optimal accuracy from 35-100% of its nominal volume, and should not be used below 10% of that volume. [6] For example, using a 1,000 μl pipette for 50 μl of liquid is not ideal, and using a 100 μl pipette will give better results. [7] Other factors like tip angle and immersion depth may also impact accuracy ...
A media dispenser or a culture media dispenser is a device for repeatedly delivering small fixed volumes (typically between 1 ml and 50 ml) of liquid such as a laboratory growth medium like molten agar or caustic or volatile solvents like toluene into a series of receptacles (Petri dishes, test tubes, Fernbach flasks, etc.).
A pipette (sometimes spelled as pipet) is a type of laboratory tool commonly used in chemistry and biology to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or ...
(These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.) A specialized example of a volumetric pipette is the microfluid pipette (capable of dispensing as little as 10 μL) designed with a circulating liquid tip that generates a self-confining volume in front of its outlet channels. [3]
A small pipette allows for more precise measurement of fluids; a larger pipette can be used to measure volumes when the accuracy of the measurement is less critical. Accordingly, pipettes vary in volume, with most measuring between 0 and 25.0 millilitres (0.00 and 0.88 imp fl oz; 0.00 and 0.85 US fl oz). [2]
Graduated cylinders are generally more accurate and precise than laboratory flasks and beakers, but they should not be used to perform volumetric analysis; [3] volumetric glassware, such as a volumetric flask or volumetric pipette, should be used, as it is even more accurate and precise. Graduated cylinders are sometimes used to measure the ...
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