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It requires boiling deparaffinized formalin fixed paraffin embedded tissue sections in either water or various buffer solutions. Due to the limited application of non-heating AR and the prevalence of heat-induced AR, the term "Antigen Retrieval" (AR) is most commonly used to refer to heat-induced AR.
In the tissue microarray technique, a hollow needle is used to remove tissue cores as small as 0.6 mm in diameter from regions of interest in paraffin-embedded tissues such as clinical biopsies or tumor samples. These tissue cores are then inserted in a recipient paraffin block in a precisely spaced, array pattern.
The substance used to embed tissue is embedding media, which is chosen depends on the category of the microscope, category of the micro tome, and category of tissue. [23] Paraffin wax, whose melting point is from 56 to 62°C, is commonly used for embedding. [22] Tissue processing - Tissue sections on slides are stained on an automated stainer
Tissue embedded within optimal cutting temperature compound (OCT), mounted on a chuck in a cryostat, and ready for section production. The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery. [1] The technical name for this procedure is ...
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a ...
A major technology of regenerative medicine is tissue engineering, [2] which has variously been defined as "an interdisciplinary field that applies the principles of engineering and the life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function", or "the creation of new tissue by the ...
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