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In microbiology, the term isolation refers to the separation of a strain from a natural, mixed population of living microbes, as present in the environment, for example in water or soil, or from living beings with skin flora, oral flora or gut flora, in order to identify the microbe(s) of interest. [1]
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology.
In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.
Cell isolation is the process of separating individual living cells from a solid block of tissue or cell suspension. While some types of cell naturally exist in a separated form (for example blood cells ), other cell types that are found in solid tissue require specific techniques to separate them into individual cells.
Cell isolation: Process of separating individual living cells from a solid block of tissue or cell suspension: Cell biology: Centrifugation: Use of centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity, and rotor speed: Cell biology, Biochemistry: CRISPR gene editing
These primary cells can then be further expanded and transferred into fresh dishes through micropropagation. Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding extracellular matrix to more accurately mimic the in vivo environment e.g. cartilage explant culture, [ 3 ] or ...
Primary isolate is a pure microbial or viral sample that has been obtained from an infected individual, rather than grown in a laboratory. In chemistry and bacteriology, the verb isolate means to obtain a pure chemical, bacteriological or viral sample. The noun 'isolate' refers to the sample itself. [citation needed]