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The yeast deletion project, formally the Saccharomyces Genome Deletion Project, is a project to create data for a near-complete collection of gene-deletion mutants of the yeast Saccharomyces cerevisiae. Each strain carries a precise deletion of one of the genes in the genome. This allows researchers to determine what each gene does by comparing ...
Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in S.cerevisiae, the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout ORF of the yeast genome (currently 4786 strains). [9]
Genode is a free and open-source software operating system (OS) framework consisting of a microkernel abstraction layer and a set of user space components. [4] [5] [6] The framework is notable as one of the few open-source operating systems not derived from a proprietary OS, such as Unix.
This category contains operating systems that are described as "free software" or "open-source software". There are multiple licenses possible for both types; licenses that specify what can and cannot be done with the software.
Singularity is a free and open-source computer program that performs operating-system-level virtualization also known as containerization. [4] One of the main uses of Singularity is to bring containers and reproducibility to scientific computing and the high-performance computing (HPC) world. [5]
In yeast, deletion strains are frequently used to assess protein stability over time with cycloheximide chases. For example, yeast strains lacking critical degradation machinery such as chaperones, E3 ligases, and vacuolar proteins are often used to determine the mechanism of degradation for a protein substrate of interest.
The human microbiota is a complex system with as many as 400 trillion microbes and bacteria interacting with each other and the host. To understand key factors in this system; a multi-scale, dynamic flux-balance analysis is proposed as FBA is classified as less computationally intensive. [12]
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