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Ethidium bromide (or homidium bromide, [2] chloride salt homidium chloride) [3] [4] is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
Bromoethane, also known as ethyl bromide, is a chemical compound of the haloalkanes group. It is abbreviated by chemists as EtBr (which is also used as an abbreviation for ethidium bromide ). This volatile compound has an ether-like odor.
Ethidium bromide This page was last edited on 4 December 2017, at 00:50 (UTC). Text is available under the Creative Commons Attribution-ShareAlike 4.0 ...
These may be metal salts containing bromide ion such as potassium bromide, or more covalent bromides of metals or nonmetals such as tantalum(V) bromide or phosphorus tribromide. See also [ edit ]
The article stated that EtBr is an abbreviation for ethidium bromide; I've always seen Et used as an abbreviation for ethyl, so EtBr would be bromoethane or ethyl bromide. I've edited accordingly. Izzycat 03:24, 27 March 2006 (UTC) Becuase it's supposed to be EthBr. Karol 09:32, 27 March 2006 (UTC)
Gel conditions are 1% agarose, 3 volt/cm, and ethidium bromide stain. A molecular-weight size marker , also referred to as a protein ladder , DNA ladder , or RNA ladder , is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis , using the principle that molecular weight is inversely ...
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The degree of unwinding varies depending on the intercalator; for example, ethidium cation (the ionic form of ethidium bromide found in aqueous solution) unwinds DNA by about 26°, whereas proflavine unwinds it by about 17°. This unwinding causes the base pairs to separate, or "rise", creating an opening of about 0.34 nm (3.4 Å).
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