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Bromothymol is used in obstetrics for detecting premature rupture of membranes. [11] Amniotic fluid typically has a pH > 7.2, bromothymol will therefore turn blue when brought in contact with fluid leaking from the amnion. As vaginal pH normally is acidic, the blue color indicates the presence of amniotic fluid.
If the test solution is of a dark colour, it is preferable to use a paper universal indicator, such as Hydrion paper. Solution: The main components of a universal indicator, in the form of a solution, are thymol blue , methyl red , bromothymol blue , and phenolphthalein .
Conversely, if a 10-fold excess of the acid occurs with respect to the base, the ratio is 1:10 and the pH is pK a − 1 or pK b − 1. For optimal accuracy, the color difference between the two species should be as clear as possible, and the narrower the pH range of the color change the better.
Bromophenol is also used as a colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein in a gel; the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in ...
acid + base → salt + water. For example: HCl + NaOH → NaCl + H 2 O. Acidimetry is the specialized analytical use of acid-base titration to determine the concentration of a basic (alkaline) substance using standard acid. This can be used for weak bases and strong bases. [8] An example of an acidimetric titration involving a strong base is as ...
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The color change to blue is useful because growth on Simmons' citrate agar is often limited and would be hard to observe if it were not for the color change. Sometimes, it is possible to detect growth on the Simmons' citrate agar without the accompanying color change to blue. This should be scored as a negative for the citrate use test. [12]
Some troops leave the battlefield injured. Others return from war with mental wounds. Yet many of the 2 million Iraq and Afghanistan veterans suffer from a condition the Defense Department refuses to acknowledge: Moral injury.