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Epiboly in zebrafish is the first coordinated cell movement, beginning at the dome stage late in the blastula period and continuing throughout gastrulation. [3] At this point the zebrafish embryo contains three portions: an epithelial monolayer known as the enveloping layer (EVL), a yolk syncytial layer (YSL) which is a membrane-enclosed group of nuclei that lie on top of the yolk cell, and ...
Fish embryos go through a process called mid-blastula transition which is observed around the tenth cell division in some fish species. Once zygotic gene transcription starts, slow cell division begins and cell movements are observable. [ 4 ]
GM fish are widely used in basic research in genetics and development. Two species of fish- zebrafish and medaka, are most commonly modified, because they have optically clear chorions (membranes in the egg), rapidly develop, and the 1-cell embryo is easy to see and microinject with transgenic DNA. [106]
Zebrafish is an important vertebrate and emerged as an important model for genetics, developmental biology, chemical biology, and regeneration. In order to establish cell culture about 200-300 zebrafish Ab strain embryos at 5-10 somite stage were dechlorinated by 300 ul/mL protease treatment and washed with PBS.
Zebrafish embryo 48 hours after fertilization. The arrows indicate four Rohon–Beard neurons. [1] Rohon–Beard cells are specialized neurons with mechanoreceptive properties. They occur during the embryonic stage of development and are found in the dorsal part of the spinal cord in fish and amphibians. [2]
Two species of fish, zebrafish (Danio rerio) and medaka (Japanese rice fish, Oryzias latipes), are most commonly modified because they have optically clear chorions (shells), develop rapidly, the 1-cell embryo is easy to see and micro-inject with transgenic DNA, and zebrafish have the capability of regenerating their organ tissues. [9]
The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family of the order Cypriniformes.Native to South Asia, [2] it is a popular aquarium fish, frequently sold under the trade name zebra danio [3] (and thus often called a "tropical fish" although it is both tropical and subtropical).
Embryo of Zebrafish undergoing cleavage. The blastodisc, also called the germinal disc, is the embryo-forming part on the yolk of the egg of an animal that undergoes discoidal meroblastic cleavage. [1] Discoidal cleavage occurs in those animals with a large proportion of yolk in their eggs, and include insects, fish, reptiles and birds. [2]