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For the Standard Methods 5210, [1] the BOD bottle “having 60 mL or greater capacity (300-mL)” is mentioned as one of the apparatus for the BOD test. A 60 mL BOD bottle is available and listed as "often convenient" by EPA (Environmental Protection Agency) Method 405.1. However, EPA Method 405.1 was written in 1974 and is no longer an EPA ...
BOD test bottles at the laboratory of a wastewater treatment plant. Biochemical oxygen demand (also known as BOD or biological oxygen demand) is an analytical parameter representing the amount of dissolved oxygen (DO) consumed by aerobic bacteria growing on the organic material present in a water sample at a specific temperature over a specific time period.
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Common cap sizes include 33-430 (33mm), 38-430 (38mm), and GL 45 (45mm). Caps range in size from narrow mouthed to wide mouthed and often a glass or plastic funnel is needed to properly fill a reagent bottle from a larger or equal sized container's mouth. Reagent bottle caps are commonly said to be "autoclavable".
The BOD test is not bottle dependent, many labs use a 500ml bottle for the samples under test if using a titration method for determination or as small as 50ml if using an electronic DO probe, but the size of the bottle is only a function of practicality and space.
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