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A 60 mL BOD bottle is available and listed as "often convenient" by EPA (Environmental Protection Agency) Method 405.1. However, EPA Method 405.1 was written in 1974 and is no longer an EPA-approved method per 40CFR Part 136.
BOD test bottles at the laboratory of a wastewater treatment plant. Biochemical oxygen demand (also known as BOD or biological oxygen demand) is an analytical parameter representing the amount of dissolved oxygen (DO) consumed by aerobic bacteria growing on the organic material present in a water sample at a specific temperature over a specific time period.
2 oz - 60 mL Amber Glass Boston Round Bottles with Gold Metal and Glass Dropper [1] A Boston round bottle, or Winchester bottle, is a strong, heavy bottle commonly used in the drug and chemical industries. It is often made of amber (brown) glass (to filter out UV light) but can also be made of plastics.
FW = Formula weight of the oxidizable compound in the sample, RMO = Ratio of the # of moles of oxygen to # of moles of oxidizable compound in their reaction to CO 2, water, and ammonia. For example, if a sample has 500 Wppm (Weight Parts per Million) of phenol: C 6 H 5 OH + 7O 2 → 6CO 2 + 3H 2 O COD = (500/94)·7·16*2 = 1192 Wppm
The difference in DO and the dilution factor are used to calculated BOD 5. The resulting number (usually reported in parts per million or milligrams per liter) is useful in determining the relative organic strength of sewage or other polluted waters. The BOD 5 test is an example of analysis that determines classes of materials in a sample.
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The BOD test is not bottle dependent, many labs use a 500ml bottle for the samples under test if using a titration method for determination or as small as 50ml if using an electronic DO probe, but the size of the bottle is only a function of practicality and space.
By comparison, typical whisky bottles weigh between 400 grams and 1,000 grams, he said.