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The tube method involves mixing a suspension of red blood cells with antisera (or plasma, for reverse grouping) in a test tube. The mixture is centrifuged to separate the cells from the reagent, and then resuspended by gently agitating the tube. If the antigen of interest is present, the red blood cells agglutinate, forming a solid clump in the ...
Acid–base and blood gases are among the few blood constituents that exhibit substantial difference between arterial and venous values. [6] Still, pH, bicarbonate and base excess show a high level of inter-method reliability between arterial and venous tests, so arterial and venous values are roughly equivalent for these. [44]
The average red blood cell contains 250 million hemoglobin molecules. [7] Hemoglobin contains a globin protein unit with four prosthetic heme groups (hence the name heme-o-globin); each heme is capable of reversibly binding with one gaseous molecule (oxygen, carbon monoxide, cyanide, etc.), [8] therefore a typical red blood cell may carry up to one billion gas molecules.
Carbonic anhydrase catalyzes the conversion of carbon dioxide and water into carbonic acid. This molecule breaks down into bicarbonate and hydrogen ions. This break down process occurs in red blood cells. Ultimately, the concentration of bicarbonate ions in the bloodstream affects the formation of the protein carbaminohemoglobin in the body. [11]
The hematocrit (/ h ɪ ˈ m æ t ə k r ɪ t /) (Ht or HCT), also known by several other names, is the volume percentage (vol%) of red blood cells (RBCs) in blood, [1] [2] measured as part of a blood test. [3] The measurement depends on the number and size of red blood cells. [3] It is normally 40.7–50.3% for males and 36.1–44.3% for ...
The parameter standard bicarbonate concentration (SBC e) is the bicarbonate concentration in the blood at a P a CO 2 of 40 mmHg (5.33 kPa), full oxygen saturation and 36 °C. [13] Reference ranges for blood tests, comparing blood content of bicarbonate (shown in blue at right) with other constituents.
The standard definition of a reference range for a particular measurement is defined as the interval between which 95% of values of a reference population fall into, in such a way that 2.5% of the time a value will be less than the lower limit of this interval, and 2.5% of the time it will be larger than the upper limit of this interval, whatever the distribution of these values.
The version with seven tests is often referred to by medical professionals in the United States as the "CHEM-7", or "SMA-7" (Sequential Multiple Analysis-7). [1] The seven parts of a CHEM-7 are tests for: Four electrolytes: sodium (Na +) [2] potassium (K +) [3] chloride (Cl −) [4] bicarbonate (HCO 3 −) or CO 2 [5] blood urea (BU), blood ...