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Ziehl–Neelsen staining is a type of narrow spectrum fungal stain. Narrow spectrum fungal stains are selective, and they can help differentiate and identify fungi. [ 10 ] The results of Ziehl–Neelsen staining is variable because many fungal cell walls are not acid fast. [ 11 ]
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue.
Franz Ziehl introduced the carbol fuchsin stain for the tubercle bacillus in 1882. With pathologist Friedrich Neelsen (1854–1898), he developed the Ziehl–Neelsen stain , [ 1 ] also known as the acid-fast stain, which is used to identify acid-fast bacteria .
Ziehl–Neelsen stain (classic and modified bleach types) [5]; Kinyoun stain; For color blind people (or in backgrounds where detecting red bacteria is difficult), Victoria blue can be substituted for carbol fuchsin and picric acid can be used as the counter stain instead of methylene blue, and the rest of the Kinyoun technique can be used.
English: This is a diagram of the basic steps of a Ziehl-Neelsen (Acid Fast) staining procedure File:Basic steps of acid fast staining procedure.svg is a vector version of this file. It should be used in place of this PDF file when not inferior.
Histological sections were stained either with Haematoxylin-Eosin (HE) (A, C–E), Ziehl-Neelsen (counterstain methylenblue) (ZN) (B) .. A: Punch biopsy with large necrotic areas, fat cell ghosts and oedema but relatively intact epidermis and dermis. B: a band of extracellular AFBs is present in a deep layer of the necrotic subcutis."
Friedrich C.A. Neelsen was born to Hans Friedrich Neelsen, deacon of the Uetersen vicarage, and his wife Bertha Sophia (née Lueders). He attended school in Uetersen and later in Altona . He studied medicine at the University of Leipzig , from which he received his doctorate at the age of 22.
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