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Log reduction. Log reduction is a measure of how thoroughly a decontamination process reduces the concentration of a contaminant. It is defined as the common logarithm of the ratio of the levels of contamination before and after the process, so an increment of 1 corresponds to a reduction in concentration by a factor of 10.
D-value (microbiology) In microbiology, in the context of a sterilization procedure, the D-value or decimal reduction time (or decimal reduction dose) is the time (or dose of an antimicrobial drug) required, at a given condition (e.g. temperature) or set of conditions, to achieve a one- log reduction, that is, to kill 90% of relevant ...
Z-value is a term used in microbial thermal death time calculations. It is the number of degrees the temperature has to be increased to achieve a tenfold (i.e. 1 log 10) reduction in the D-value. The D-value of an organism is the time required in a given medium, at a given temperature, for a ten-fold reduction in the number of organisms.
Sterility assurance level. In microbiology, sterility assurance level (SAL) is the probability that a single unit that has been subjected to sterilization nevertheless remains nonsterile. It is never possible to prove that all organisms have been destroyed, as the likelihood of survival of an individual microorganism is never zero.
This is usually done by thermal processing and finding ways to reduce the number of bacteria in the product. Time-temperature measurements of bacterial reduction is determined by a D-value, meaning how long it would take to reduce the bacterial population by 90% or one log 10 at a given temperature.
Bacterial growth. Growth is shown as L = log (numbers) where numbers is the number of colony forming units per ml, versus T (time.) Bacterial growth is proliferation of bacterium into two daughter cells, in a process called binary fission. Providing no mutation event occurs, the resulting daughter cells are genetically identical to the original ...
A low-pressure mercury-vapor discharge tube floods the inside of a biosafety cabinet with shortwave UV light when not in use, killing microbes on irradiated surfaces. Ultraviolet germicidal irradiation (UVGI) is a disinfection technique employing ultraviolet (UV) light, particularly UV-C (180–280 nm), to kill or inactivate microorganisms.
(A log 10 reduction between 6 and 7 means that 1 bacterium out of 1 million (10 6) to 10 million (10 7) bacteria survive the treatment.) The Codex Alimentarius Code of Hygienic Practice for Milk notes that milk pasteurization is designed to achieve at least a 5 log 10 reduction of Coxiella burnetii. [48]