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DNA profiling is a forensictechnique in criminal investigations, comparing criminal suspects' profiles to DNA evidence so as to assess the likelihood of their involvement in the crime. [1][2]It is also used in paternity testing,[3]to establish immigration eligibility,[4]and in genealogicaland medical research.
Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by KeyGene, [1] AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments.
DNA footprinting is a method of investigating the sequence specificity of DNA -binding proteins in vitro. This technique can be used to study protein-DNA interactions both outside and within cells. The regulation of transcription has been studied extensively, and yet there is still much that is unknown.
Microfluidic Sanger sequencing is a lab-on-a-chip application for DNA sequencing, in which the Sanger sequencing steps (thermal cycling, sample purification, and capillary electrophoresis) are integrated on a wafer-scale chip using nanoliter-scale sample volumes.
Alec Jeffreys. Sir Alec John Jeffreys, CH FRS MAE [ 7 ] (born 9 January 1950) [ 6 ] is a British geneticist known for developing techniques for genetic fingerprinting and DNA profiling which are now used worldwide in forensic science to assist police detective work and to resolve paternity and immigration disputes. [ 5 ][ 8 ][ 9 ]
In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, populations, or species or to pinpoint the locations of genes within a sequence. The term may refer to a polymorphism itself, as detected through the differing locations of ...
The fundamental autosomal number or autosomal fundamental number, FNa[36]or AN,[37]of a karyotype is the number of visible major chromosomal arms per set of autosomes(non-sex-linked chromosomes). Ploidy. [edit] Ploidyis the number of complete sets of chromosomes in a cell. Polyploidy, where there are more than two sets of homologous chromosomes ...
Centre for DNA Fingerprinting and Diagnostics (CDFD), Uppal campus. CDFD was conceptualised by then CCMB director Lalji Singh. It evolved into its current form of a modern institution encompassing both basic and applied research in diverse areas of modern biology under its founder director, Seyed E. Hasnain (former vice-chancellor of the University of Hyderabad), who pursued this aim ...