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The following table presents pK a values at 20 °C. Values change by about 0.01 per degree of temperature. [1] [3] Good's original 1966 paper had two older buffers (marked with italics) for comparison. In 1972 Good published a second list with three more buffers, and five more were added in 1980.
There are many different ways to prepare PBS solutions, common ones are Dulbecco's phosphate-buffered saline (DPBS) [2] and the Cold Spring Harbor protocol. [3] Some formulations of DPBS do not contain potassium and magnesium, while other ones contain calcium and/or magnesium (depending on whether or not the buffer is used on live or fixed tissue: the latter does not require CaCl 2 or MgCl 2).
Monopotassium phosphate (MKP) (also, potassium dihydrogen phosphate, KDP, or monobasic potassium phosphate) is the inorganic compound with the formula KH 2 PO 4. Together with dipotassium phosphate (K 2 HPO 4. (H 2 O) x) it is often used as a fertilizer, food additive, and buffering agent.
A buffer solution is a solution where the pH does not change significantly on dilution or if an acid or base is added at constant temperature. [1] Its pH changes very little when a small amount of strong acid or base is added to it. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical ...
Potassium phosphate is a generic term for the salts of potassium and phosphate ions including: [1] Monopotassium phosphate (KH 2 PO 4) (Molar mass approx: 136 g/mol) Dipotassium phosphate (K 2 HPO 4) (Molar mass approx: 174 g/mol) Tripotassium phosphate (K 3 PO 4) (Molar mass approx: 212.27 g/mol) As food additives, potassium phosphates have ...
The tables below provides information on the variation of solubility of different substances (mostly inorganic compounds) in water with temperature, at one atmosphere pressure. Units of solubility are given in grams of substance per 100 millilitres of water (g/(100 mL)), unless shown otherwise.
McIlvaine buffer is a buffer solution composed of citric acid and disodium hydrogen phosphate, also known as citrate-phosphate buffer. It was introduced in 1921 by the United States agronomist Theodore Clinton McIlvaine (1875–1959) from West Virginia University , and it can be prepared in pH 2.2 to 8 by mixing two stock solutions.
Tripotassium phosphate, also called tribasic potassium phosphate [3] is a water-soluble salt with the chemical formula K 3 PO 4. (H 2 O) x (x = 0, 3, 7, 9). [ 4 ] Tripotassium phosphate is basic: a 1% aqueous solution has a pH of 11.8.