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Saline solution for irrigation. Normal saline (NSS, NS or N/S) is the commonly used phrase for a solution of 0.90% w/v of NaCl, 308 mOsm/L or 9.0 g per liter. Less commonly, this solution is referred to as physiological saline or isotonic saline (because it is approximately isotonic to blood serum, which makes it a physiologically normal solution).
Depiction of a red blood cell in an isotonic solution. A solution is isotonic when its effective osmole concentration is the same as that of another solution. In biology, the solutions on either side of a cell membrane are isotonic if the concentration of solutes outside the cell is equal to the concentration of solutes inside the cell.
The most commonly used crystalloid fluid is normal saline, a solution of sodium chloride at 0.9% concentration, which is close to the concentration in the blood . [3] Ringer's lactate or Ringer's acetate is another isotonic solution often used for large-volume fluid replacement. The choice of fluids may also depend on the chemical properties of ...
There are many different ways to prepare PBS solutions, common ones are Dulbecco's phosphate-buffered saline (DPBS) [2] and the Cold Spring Harbor protocol. [3] Some formulations of DPBS do not contain potassium and magnesium, while other ones contain calcium and/or magnesium (depending on whether or not the buffer is used on live or fixed tissue: the latter does not require CaCl 2 or MgCl 2).
Before such use other substances generally must be added to make the solution isotonic. [3] Isotonic solutions containing water for injection can be given by injection into a vein, muscle, or under the skin. [4] A non-sterile version may be used in manufacturing with sterilization occurring later in the production process. [5]
Balanced salt solutions are used for washing tissues and cells and are usually combined with other agents to treat the tissues and cells. They provide the cells with water and inorganic ions, while maintaining a physiological pH and osmotic pressure. [2] Sometimes glucose is added as an energy source and phenol red is used as a pH indicator.
Medical uses [ edit ] To prepare an isotonic saline solution for nasal rinsing, approx. 0.25 liter lukewarm, sterilized drinking water and 2.5 ml of table salt (about half a level teaspoon ) is required.
Isotonic solutions have the same salt concentration as the human body, whereas hypertonic solutions have a higher salt content and hypotonic solutions have a lower salt content. Isotonic saline nasal sprays are commonly used in infants and children to wash out the thick mucus from the nose in case of allergic rhinitis. Hypertonic solutions may ...