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Major applications include blood smears, bone marrow aspirates, semen analysis and cytology of various body fluids including urine and cerebrospinal fluid. [7] [8] Microbiologic agents, such as bacteria and fungi, also appear more easily in Diff-Quik. [3] This is useful for the detection of for example Helicobacter pylori from gastric and ...
Brushes used to collect samples for cytology. Liquid-based cytology is a method of preparing samples for examination in cytopathology.The sample is collected, normally by a small brush, in the same way as for a conventional smear test, but rather than the smear being transferred directly to a microscope slide, the sample is deposited into a small bottle of preservative liquid.
Alsever's solution is a saline liquid used to prevent coagulation of blood. [ 1 ] [ 2 ] It is composed of 2.05% dextrose , 0.8% sodium citrate , 0.055% citric acid , and 0.42% sodium chloride . For usage, an equal volume of blood is gently, but thoroughly, mixed with the solution.
The third staining solution is composed of three dyes, Eosin Y, Light Green SF yellowish, and Bismarck brown Y in 95% ethyl alcohol with a small amount of phosphotungstic acid and lithium carbonate. [ 12 ] [ 2 ] This solution, designated EA, followed by a number that denotes the proportion of the dyes, other formulations include EA-36, EA-50 ...
Carnoy's solution is a fixative composed of 60% ethanol, 30% chloroform and 10% glacial acetic acid, 1 gram of ferric chloride. [ 1 ] [ 2 ] Carnoy's solution is also the name of a different fixation composed of ethanol and glacial acetic acid (3:1).
Giemsa's solution is a mixture of methylene blue, eosin, and Azure B. The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared ...
There are many different ways to prepare PBS solutions, common ones are Dulbecco's phosphate-buffered saline (DPBS) [2] and the Cold Spring Harbor protocol. [3] Some formulations of DPBS do not contain potassium and magnesium, while other ones contain calcium and/or magnesium (depending on whether or not the buffer is used on live or fixed tissue: the latter does not require CaCl 2 or MgCl 2).
Sample collection for thin-prep-cytology from the cervix uteri of a 39-years-old multiparous woman. The cervical brush is visible just before entering the cervix uteri. Brush utilized in cervical screening exams to collect samples. The procedures for testing women using Pap smear, liquid-based cytology, or HPV testing are similar.