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Brushes used to collect samples for cytology. Liquid-based cytology is a method of preparing samples for examination in cytopathology.The sample is collected, normally by a small brush, in the same way as for a conventional smear test, but rather than the smear being transferred directly to a microscope slide, the sample is deposited into a small bottle of preservative liquid.
In hemocytometry, Türk's solution (or Türk's fluid) is a hematological stain (either crystal violet or aqueous methylene blue) prepared in 99% acetic acid (glacial) [1] and distilled water. The solution destroys the red blood cells and platelets within a blood sample (acetic acid being the main lyzing agent ), and stains the nuclei of the ...
Major applications include blood smears, bone marrow aspirates, semen analysis and cytology of various body fluids including urine and cerebrospinal fluid. [7] [8] Microbiologic agents, such as bacteria and fungi, also appear more easily in Diff-Quik. [3] This is useful for the detection of for example Helicobacter pylori from gastric and ...
Hanks' salts is a collective group of salts rich in bicarbonate ions, formulated in 1940 by the microbiologist John H. Hanks. [1] Typically, they are used as a buffer system in cell culture media and aid in maintaining the optimum physiological pH (roughly 7.0–7.4) for cellular growth.
The third staining solution is composed of three dyes, Eosin Y, Light Green SF yellowish, and Bismarck brown Y in 95% ethyl alcohol with a small amount of phosphotungstic acid and lithium carbonate. [ 12 ] [ 2 ] This solution, designated EA, followed by a number that denotes the proportion of the dyes, other formulations include EA-36, EA-50 ...
Giemsa's solution is a mixture of methylene blue, eosin, and Azure B. The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared ...
To prepare 1 liter of TSB, the following ingredients are dissolved under gentle heat. Adjustments to pH should be made using 1N HCl or 1N NaOH to reach a final target pH of 7.3 ± 0.2 at 25°C. The solution is then autoclaved for 15 minutes at 121°C. [3] Tryptic Soy Agar contains per liter: 17 g pancreatic digest of casein; 3 g peptic digest ...
There are many different ways to prepare PBS solutions, common ones are Dulbecco's phosphate-buffered saline (DPBS) [2] and the Cold Spring Harbor protocol. [3] Some formulations of DPBS do not contain potassium and magnesium, while other ones contain calcium and/or magnesium (depending on whether or not the buffer is used on live or fixed tissue: the latter does not require CaCl 2 or MgCl 2).