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TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.. Compared to the other two most common agents used for this purpose (dithiothreitol and β-mercaptoethanol), TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent (in the sense that TCEP does not ...
2-Mercaptoethanol is often used interchangeably with dithiothreitol (DTT) or the odorless tris(2-carboxyethyl)phosphine (TCEP) in biological applications. Although 2-mercaptoethanol has a higher volatility than DTT, it is more stable: 2-mercaptoethanol's half-life is more than 100 hours at pH 6.5 and 4 hours at pH 8.5; DTT's half-life is 40 ...
A valve stem is a self-contained valve that opens to admit gas to a chamber (such as air to inflate a tire), [1] and is then automatically closed and kept sealed by the pressure in the chamber, or a spring, [2] [3] or both, to prevent the gas from escaping.
Several types of valve connections for propane, butane, and LPG containers exist for transport and storage, sometimes with overlapping usage and applications, and there are major differences in usage between different countries. Even within a single country more than one type can be in use for a specific application.
Dithiobutylamine (DTBA) is a reducing agent intended as an alternative for DTT in biochemical uses. It was designed to be easily synthesized in non-racemic form, to have a lower pKa (allowing more effective reduction at neutral pH), and to have a low disulfide E°′ reduction potential. [1]
Category 2 seal are intended for API-610 This category is applicable for temperatures between –40 °F and 750 °F (-40 °C and 400 °C) and pressures to 615 PSI (42 bar). Category 3 seals are essentially the original seals of 1st Edition and are also intended for API-610 pumps. Category 3 seals are intended for the most demanding applications.
Radial shaft seals, also known as lip seals, are used to seal rotary elements, such as a shaft or rotating bore. Common examples include strut seals, hydraulic pump seals, axle seals, power steering seals, and valve stem seals.
Dithiothreitol (DTT) is an organosulfur compound with the formula (CH(OH)CH 2 SH) 2. A colorless compound, it is classified as a dithiol and a diol . DTT is redox reagent also known as Cleland's reagent , after W. Wallace Cleland . [ 2 ]
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