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FCCS is an extension of the fluorescence correlation spectroscopy (FCS) method that uses two fluorescent molecules instead of one that emits different colours. The technique measures coincident green and red intensity fluctuations of distinct molecules that correlate if green and red labelled particles move together through a predefined confocal volume. [2]
A basic diagram of a fluorescence correlation spectroscopy instrument. The typical FCS setup consists of a laser line (wavelengths ranging typically from 405–633 nm (), and from 690–1100 nm (pulsed)), which is reflected into a microscope objective by a dichroic mirror.
A human visual system model (HVS model) is used by image processing, video processing and computer vision experts to deal with biological and psychological processes that are not yet fully understood. Such a model is used to simplify the behaviors of what is a very complex system. As our knowledge of the true visual system improves, the model ...
Human Design is a pseudoscientific [1] [2] new age practice, described as a holistic self-knowledge system. [3] It combines astrology , the Chinese I Ching , Judaic Kabbalah , Vedic philosophy and modern physics .
In molecular spectroscopy, a Jablonski diagram is a diagram that illustrates the electronic states and often the vibrational levels of a molecule, and also the transitions between them. The states are arranged vertically by energy and grouped horizontally by spin multiplicity . [ 1 ]
Diagram of the design of a STED device. The double laser design allows for excitation and stimulated emission to be used together for STED. In traditional microscopy, the resolution that can be obtained is limited by the diffraction of light. Ernst Abbe developed an equation to describe this limit. The equation is:
Recent developments in the design and quality of crystal optics have allowed for some EXAFS measurements to take place in a lab setting, [3] where the tunable x-ray source is achieved via a Rowland circle geometry. While experiments requiring high x-ray flux or specialized sample environments can still only be performed at synchrotron ...
Multiple-pass or long path absorption cells are commonly used in spectroscopy to measure low-concentration components or to observe weak spectra in gases or liquids. Several important advances were made in this area beginning in the 1930s, and research into a wide range of applications continues to the present day.