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By convention, the coding strand is the strand used when displaying a DNA sequence. It is presented in the 5' to 3' direction. Wherever a gene exists on a DNA molecule, one strand is the coding strand (or sense strand), and the other is the noncoding strand (also called the antisense strand, [3] anticoding strand, template strand or transcribed ...
Information and communications technology (ICT) is an extensional term for information technology (IT) that stresses the role of unified communications [1] and the integration of telecommunications (telephone lines and wireless signals) and computers, as well as necessary enterprise software, middleware, storage and audiovisual, that enable users to access, store, transmit, understand and ...
[1] Ribbon diagrams are simple yet powerful, expressing the visual basics of a molecular structure (twist, fold and unfold). This method has successfully portrayed the overall organization of protein structures, reflecting their three-dimensional nature and allowing better understanding of these complex objects both by expert structural ...
The non-template (sense) strand of DNA is called the coding strand, because its sequence is the same as the newly created RNA transcript (except for the substitution of uracil for thymine). This is the strand that is used by convention when presenting a DNA sequence.
Despite DNA being a double-stranded molecule, only one of the strands acts as a template for pre-mRNA synthesis; this strand is known as the template strand. The other DNA strand (which is complementary to the template strand) is known as the coding strand. [6]
The Pribnow box has a 6 bp region centered around the -10 position and an 8-12 bp sequence around the -35 region that are both conserved. [10] A CAAT box (also CAT box) is a region of nucleotides with the following consensus sequence: 5’ GGCCAATCT 3’. The CAAT box is located about 75-80 bases upstream of the transcription initiation site ...
Given that there are four choices for each base in the strand and a 20bp - 22bp length for a mi/siRNA, that leads to more than 1 × 10 12 possible combinations. Given that the human genome is ~3.1 billion bases in length, [ 11 ] this means that each miRNA should only find a match once in the entire human genome by accident.
Once the DNA strand has been read, the strand that was just added is washed away. Then, the index 1 primer attaches, polymerizes the index 1 sequence, and is washed away. The strand forms a bridge again, and the 3' end of the DNA strand attaches to an oligo on the flow cell. The index 2 primer attaches, polymerizes the sequence, and is washed away.