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It was developed in 1953 by Hugh and Leifson to be utilized in microbiology to determine the way a microorganism metabolizes a carbohydrate such as glucose (dextrose). [1] OF-glucose deeps contain glucose as a carbohydrate, peptones, bromothymol blue indicator for Hugh-Leifson's OF medium or phenol red for King's OF medium, and 0.5% agar.
Bromothymol blue (also known as bromothymol sulfone phthalein and BTB) is a pH indicator. It is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). A common use is for measuring the presence of carbonic acid in a liquid.
Bromothymol blue is the indicator used in the agar, it changes to yellow in case of acid production during fermentation of lactose or changes to deep blue in case of alkalinization. Lactose-positive bacteria build yellow colonies. Bacteria which decarboxylate L-cystine cause an alkaline reaction and build deep blue colonies. [1]
Universal indicator components Indicator Low pH colour Transition pH range High pH colour Thymol blue (first transition) Red 1.2 – 2.8 Yellow Methyl orange: Red 3.2 – 4.4 Yellow Methyl red: Red 4.8 – 6.0 Yellow Bromothymol blue: Yellow 6.0 – 7.6 Blue Thymol blue (second transition) Yellow 8.0 – 9.6 Blue Phenolphthalein: Colourless
The increase in pH then causes color change in the bromothymol blue indicator, turning it blue. Under neutral conditions the medium remains a green color. The color change to blue is useful because growth on Simmons' citrate agar is often limited and would be hard to observe if it were not for the color change.
This category contains articles about pH indicators: chemical compounds which change colour or fluorescence in response to changes in pH Wikimedia Commons has media related to PH indicators . Subcategories
Sucrose fermentation produces acid, which converts the colour of bromothymol blue or thymol blue. Two dyes rather than one make the medium produce an array of yellow, green, or blue so that differentiating among various Vibrio species is possible.
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