enow.com Web Search

Search results

  1. Results from the WOW.Com Content Network
  2. RNA interference - Wikipedia

    en.wikipedia.org/wiki/RNA_interference

    Specialized laboratory techniques have also been developed to improve the utility of RNAi in mammalian systems by avoiding the direct introduction of siRNA, for example, by stable transfection with a plasmid encoding the appropriate sequence from which siRNAs can be transcribed, [109] or by more elaborate lentiviral vector systems allowing the ...

  3. RNA silencing - Wikipedia

    en.wikipedia.org/wiki/RNA_silencing

    RNA silencing describes several mechanistically related pathways which are involved in controlling and regulating gene expression. [5] [6] [7] RNA silencing pathways are associated with the regulatory activity of small non-coding RNAs (approximately 20–30 nucleotides in length) that function as factors involved in inactivating homologous sequences, promoting endonuclease activity ...

  4. DNA-directed RNA interference - Wikipedia

    en.wikipedia.org/wiki/DNA-directed_RNA_interference

    DNA-directed RNA interference (ddRNAi) is a gene-silencing technique that utilizes DNA constructs to activate a cell's endogenous RNA interference (RNAi) pathways. DNA constructs are designed to express self-complementary double-stranded RNAs, typically short-hairpin RNAs (shRNA), that bring about the silencing of a target gene or genes once processed. [1]

  5. RNAI - Wikipedia

    en.wikipedia.org/wiki/RNA_I

    RNAI is a non-coding RNA that is an antisense repressor of the replication of some E. coli plasmids, including ColE1. Plasmid replication is usually initiated by RNAII, [1] which acts as a primer by binding to its template DNA. The complementary RNAI binds RNAII prohibiting it from its initiation role.

  6. Argonaute - Wikipedia

    en.wikipedia.org/wiki/Argonaute

    The other single-stranded RNA, named the passenger strand, is degraded during the RNA-induced silencing complex process. [7] Once the Argonaute is associated with the small RNA, the enzymatic activity conferred by the PIWI domain cleaves only the passenger strand of the small interfering RNA. RNA strand separation and incorporation into the ...

  7. Small interfering RNA - Wikipedia

    en.wikipedia.org/wiki/Small_interfering_RNA

    Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded non-coding RNA molecules, typically 20–24 base pairs in length, similar to microRNA (miRNA), and operating within the RNA interference (RNAi) pathway.

  8. Microarray analysis techniques - Wikipedia

    en.wikipedia.org/wiki/Microarray_analysis_techniques

    Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail. Microarray analysis techniques are used in interpreting the data generated from experiments on DNA (Gene chip analysis), RNA, and protein microarrays, which allow researchers to investigate the expression state of a large number of genes – in many cases, an organism's entire genome – in a ...

  9. Nuclease protection assay - Wikipedia

    en.wikipedia.org/wiki/Nuclease_protection_assay

    Nuclease protection assay is a laboratory technique used in biochemistry and genetics to identify individual RNA molecules in a heterogeneous RNA sample extracted from cells. The technique can identify one or more RNA molecules of known sequence even at low total concentration. The extracted RNA is first mixed with antisense RNA or DNA probes ...