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N-Hydroxysuccinimide (NHS) is an organic compound with the formula (CH 2 CO) 2 NOH. It is a white solid that is used as a reagent for preparing active esters in peptide synthesis. It can be synthesized by heating succinic anhydride with hydroxylamine or hydroxylamine hydrochloride. [2]
Hantzsch ester refers to an organic compound with the formula HN(MeC=C(CO 2 Et)) 2 CH 2 where Me = methyl (CH 3) and Et = ethyl (C 2 H 5).It is a light yellow solid. The compound is a 1,4-dihydropyridine.
The ester linkage is formed between the N-hydroxyphthalimide and a carboxylic acid by elimination of water, the coupling achieved with N,N′-dicyclohexylcarbodiimide (DCC). For peptide synthesis, the N-terminus of the growing peptide is protected with tert-butyloxycarbonyl while its C-terminus (Z–NH–CH(R)–COOH) is coupled to N ...
Common uses for this carbodiimide include peptide synthesis, protein crosslinking to nucleic acids, but also in the preparation of immunoconjugates. EDC is often used in combination with N-hydroxysuccinimide (NHS) for the immobilisation of large biomolecules. Recent work has also used EDC to assess the structure state of uracil nucleobases in RNA.
Ecoinformatics – research dedicated to the development and dissemination of technological tools that facilitate analysis and synthesis in ecology. Conservation and Resource Management – research focused on areas such as conservation biology and the explicit application of science to natural resource management and policy decision making.
Aside from the acid chloride substrate, three reagents are required: diazomethane, water, and a metal catalyst. Each has been well investigated. The diazomethane is required in excess so as to react with the HCl formed previously. [2] Not taking diazomethane in excess results in HCl reacting with the diazoketone to form chloromethyl ketone and ...
A synthesis of 1,4-dihydropyridines in aqueous micelles catalyzed by PTSA under ultrasonic irradiation. Using condensation of benzaldehyde , ethyl acetoacetate and ammonium acetate as a model, experiments have proven that when catalyzed by p-toluenesulfonic acid (PTSA) under ultrasonic irradiation, the reaction can have a product yield of 96% ...
Pentafluorophenyl (PFP) esters are chemical compounds with the generic formula RC(O)OC 6 F 5. They are active esters derived from pentafluorophenol (HOC 6 F 5 ). PFP esters are useful for attaching fluorophores such as fluorescein [ 1 ] or haptens [ 2 ] to primary amines in biomolecules.