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Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment, it can be intensely fluorescent, with varying colors from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages).
Nile red (also known as Nile blue oxazone) is formed by boiling Nile blue with sulfuric acid. This produces a mix of Nile red and Nile blue. Nile red is a lipophilic stain; it will accumulate in lipid globules inside cells, staining them red. Nile red can be used with living cells.
The lipid content of the macrophages can be demonstrated using a lipid targeting stain like Oil Red O or Nile red. [ 2 ] [ 4 ] [ 5 ] [ 6 ] Increased levels of lipid-laden alveolar macrophages are associated with various respiratory conditions, including chronic smoking, [ 3 ] gastroesophageal reflux , lipoid pneumonia , fat embolism , [ 7 ...
Detection usually involves the staining of sections of aortic sinus or artery with Oil Red O (ORO) followed by computer imaging and analysis; or from Nile Red Staining. In addition, fluorescent microscopy or flow cytometry can be used to detect OxLDL uptake when OxLDL has been labeled with 1,1′-dioctadecyl-3,3,3′3′-tetra-methylindocyanide ...
Oil Red O (Solvent Red 27, Sudan Red 5B, C.I. 26125, C 26 H 24 N 4 O) is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a red powder with an absorbance maximum at 518 nanometers. [1]
They settled on nile red, a compound for dyeing used in biochemistry, as it sounded good and did not sound much like a chemical. A second channel, NileBlue, was created in 2016 to showcase more casual projects than the main channel, and a shorts channel, NileRed 2 (formerly NileRed Shorts), was created in 2021.
Category: Vital stains. ... Nile blue; Nile red; S. Safranin; T. Trypan blue This page was last edited on 30 March 2013, at 19:40 (UTC). Text is available under ...
Nile blue (or Nile blue A) is a stain used in biology and histology. It may be used with live or fixed cells, and imparts a blue colour to cell nuclei . It may also be used in conjunction with fluorescence microscopy to stain for the presence of polyhydroxybutyrate granules in prokaryotic or eukaryotic cells.