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Peptide hydrogel formation shown by the inverted vial method. A hydrogel is a biphasic material, a mixture of porous and permeable solids and at least 10% of water or other interstitial fluid. [1] [2] The solid phase is a water insoluble three dimensional network of polymers, having absorbed a large amount of water or biological fluids.
Self-healing hydrogels are a specialized type of polymer hydrogel.A hydrogel is a macromolecular polymer gel constructed of a network of crosslinked polymer chains. Hydrogels are synthesized from hydrophilic monomers by either chain or step growth, along with a functional crosslinker to promote network formation.
The schematic summary of the production method. To produce hydrogel fiber, the solidification of the pregel solution is the most important step. The pregel solution needs to be solidified while maintaining its fibrous shape. To achieve this, several methods based on chemical crosslinking, phase change, rheological property change have been ...
Specifically, hydrogels can be designed to release drugs or other agents in response to physical characteristics of the environment like temperature and pH. [12] The responsiveness of hydrogels is a result of their molecular structure and polymer networks. [12] Hydrogel nanoparticles have a promising future in the drug delivery field.
For investigating the properties of polymer samples in particular, GPC can be very advantageous. GPC provides a more convenient method of determining the molecular weights of polymers. In fact most samples can be thoroughly analyzed in an hour or less. [17] Other methods used in the past were fractional extraction and fractional precipitation.
A common protocol used in the past for zymography of α-amylase activity was the so-called starch film protocol of W.W. Doane. Here a native PAGE gel was run to separate the proteins in a homogenate. Subsequently, a thin gel with starch dissolved (or more properly, suspended) in it was overlaid for a period of time on top of the original gel. [6]
Proteins in the gel are fixed by acetic acid and simultaneously stained. The excess dye incorporated into the gel can be removed by destaining with the same solution without the dye. The proteins are detected as blue bands on a clear background. [16] [17] When more sensitive method than staining by Coomassie is needed, silver staining is ...
Biophysical chemistry is a physical science that uses the concepts of physics and physical chemistry for the study of biological systems. [1] The most common feature of the research in this subject is to seek an explanation of the various phenomena in biological systems in terms of either the molecules that make up the system or the supra-molecular structure of these systems. [2]
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