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The United States Department of Veterans Affairs was an early adopter of a standardized approach to incorporating visible light images into the electronic medical record [7]. Increasingly, visible light imaging is being deployed beyond individual departments, as part of a trend referred to as Enterprise Imaging [ 8 ] .
Medical optical imaging is the use of light as an investigational imaging technique for medical applications, pioneered by American Physical Chemist Britton Chance. Examples include optical microscopy , spectroscopy , endoscopy , scanning laser ophthalmoscopy , laser Doppler imaging , optical coherence tomography , and transdermal optical imaging .
Radiant energy outside the visible spectrum does not contribute to photometric quantities at all, so for example a 1000 watt space heater may put out a great deal of radiant flux (1000 watts, in fact), but as a light source it puts out very few lumens (because most of the energy is in the infrared, leaving only a dim red glow in the visible).
Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
Spectral imaging may use the infrared, the visible spectrum, the ultraviolet, x-rays, or some combination of the above. It may include the acquisition of image data in visible and non-visible bands simultaneously, illumination from outside the visible range, or the use of optical filters to capture a specific spectral range. It is also possible ...
The near-infrared (NIR) window (also known as optical window or therapeutic window) defines the range of wavelengths from 650 to 1350 nanometre (nm) where light has its maximum depth of penetration in tissue. [1] Within the NIR window, scattering is the most dominant light-tissue interaction, and therefore the propagating light becomes diffused ...
Many cuvettes have a light path of 10 mm (0.39 in), which simplifies calculation of the coefficient of absorption. Most cuvettes have two transparent sides opposite one another so the spectrophotometer light can pass through, although some tests use reflection so only need a single transparent side.
Bright-field microscopy is a standard light-microscopy technique, and therefore magnification is limited by the resolving power possible with the wavelength of visible light. The practical limit to magnification with a light microscope is around 1300×.