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  2. Promoter (genetics) - Wikipedia

    en.wikipedia.org/wiki/Promoter_(genetics)

    In genetics, a promoter is a sequence of DNA to which proteins bind to initiate transcription of a single RNA transcript from the DNA downstream of the promoter. The RNA transcript may encode a protein ( mRNA ), or can have a function in and of itself, such as tRNA or rRNA .

  3. Promoter activity - Wikipedia

    en.wikipedia.org/wiki/Promoter_activity

    Promoter activity of the P-RM and P-R promoters vs RNA polymerase concentration in the enterobacteriophage lambda [1]. Promoter activity is a term that encompasses several meanings around the process of gene expression from regulatory sequences —promoters [2] and enhancers. [3]

  4. CAG promoter - Wikipedia

    en.wikipedia.org/wiki/CAG_promoter

    The CAG promoter is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian expression vectors. [1] [2] CAG promoter was constructed in the lab of Dr Jun-ichi Miyazaki [3] [4] from the following sequences: (C) the cytomegalovirus (CMV) early enhancer element,

  5. General transcription factor - Wikipedia

    en.wikipedia.org/wiki/General_transcription_factor

    A sigma factor is a protein needed only for initiation of RNA synthesis in bacteria. [12] Sigma factors provide promoter recognition specificity to the RNA polymerase (RNAP) and contribute to DNA strand separation, then dissociating from the RNA polymerase core enzyme following transcription initiation. [13]

  6. Transcription preinitiation complex - Wikipedia

    en.wikipedia.org/wiki/Transcription_pre...

    Transcription preinitiation complex, represented by the central cluster of proteins, causes RNA polymerase to bind to target DNA site. The PIC is able to bind both the promoter sequence near the gene to be transcribed and an enhancer sequence in a different part of the genome, allowing enhancer sequences to regulate a gene distant from it.

  7. lacUV5 - Wikipedia

    en.wikipedia.org/wiki/LacUV5

    The lacUV5 promoter is a mutated promoter from the Escherichia coli lac operon which is used in molecular biology to drive gene expression on a plasmid. lacUV5 is very similar to the classical lac promoter, containing just 2 base pair mutations in the -10 hexamer region, compared to the lac promoter. [1]

  8. Expression vector - Wikipedia

    en.wikipedia.org/wiki/Expression_vector

    The vector is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. [1] The goal of a well-designed expression vector is the efficient production of protein, and this may be achieved by the production of significant amount of stable ...

  9. Mediator (coactivator) - Wikipedia

    en.wikipedia.org/wiki/Mediator_(coactivator)

    [c] For example, after binding the enhancer and core promoter, the mediator complex undergoes a compositional change in which the kinase module dissociates from the complex to allow association with RNA polymerase II and transcriptional activation. [6] The Mediator complex is located within the cell nucleus.

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