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The lactose operon (lac operon) is an operon required for the transport and metabolism of lactose in E. coli and many other enteric bacteria.Although glucose is the preferred carbon source for most enteric bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available through the activity of β-galactosidase. [1]
The lac operon in the prokaryote E. coli consists of genes that produce enzymes to break down lactose. Its operon is an example of a prokaryotic silencer. The three functional genes in this operon are lacZ, lacY, and lacA. [6] The repressor gene, lacI, will produce the repressor protein LacI which is under allosteric regulation.
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A structural gene is a gene that codes for any RNA or protein product other than a regulatory factor (i.e. regulatory protein).A term derived from the lac operon, structural genes are typically viewed as those containing sequences of DNA corresponding to the amino acids of a protein that will be produced, as long as said protein does not function to regulate gene expression.
The lacZYA operon houses genes encoding proteins needed for lactose breakdown. [2] The lacI gene codes for a protein called "the repressor" or "the lac repressor", which functions to repressor of the lac operon. [2] The gene lacI is situated immediately upstream of lacZYA but is transcribed from a lacI promoter. [2]
Operon; Usage on fa.wikipedia.org سرکوبگر; Usage on fr.wikipedia.org Régulation de l'expression des gènes; Usage on gd.wikipedia.org 9 an Gearran; Uicipeid:San latha an-diugh/9 dhen Ghearran; Uicipeid:San latha an-diugh/an Gearran; Usage on gl.wikipedia.org Promotor (xenética) Regulación da expresión xénica; Represor; Operón ...
The enzyme's role in the classical E.coli lac operon remains unclear. [1] [3] However, the enzyme's cellular role may be to detoxify non-metabolizable pyranosides by acetylating them and preventing their reentry into the cell. [1] [4]
Taking advantage of an operon fusion placing the lac operon (responsible for producing beta-galactosidase, a protein which degrades lactose) under the control of an SOS-related protein, a simple colorimetric assay for genotoxicity is possible.