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The measurement of an exponential bacterial growth curve in batch culture was traditionally a part of the training of all microbiologists; the basic means requires bacterial enumeration (cell counting) by direct and individual (microscopic, flow cytometry [1]), direct and bulk (biomass), indirect and individual (colony counting), or indirect ...
Figure 1: A bi-phasic bacterial growth curve.. A growth curve is an empirical model of the evolution of a quantity over time. Growth curves are widely used in biology for quantities such as population size or biomass (in population ecology and demography, for population growth analysis), individual body height or biomass (in physiology, for growth analysis of individuals).
A portable embedded system for microbial concentration measurement in liquid and semi-liquid media using impedance microbiology has been recently proposed. [16] [17] The system is composed of a thermoregulated incubation chamber where the sample under test is stored and a controller for thermoregulation and impedance measurements.
A diauxic growth curve refers to the growth curve generated by an organism which has two growth peaks. The theory behind the diauxic growth curve stems from Jacques Monod's Ph.D. research in 1940. A simple example involves the bacterium Escherichia coli ( E. coli ), the best understood bacterium.
The method of enumeration [9] of surviving cells used by VCC is termed quantitative growth kinetics (QGK). It relates the kinetic time taken for the turbidity of a bacterial batch microbiological culture in a well of a 96-well microplate to reach a threshold difference in turbidity to a 10-fold dilution series of calibration growth curves.
Incubation follows a growth curve variable for every microorganism. Cultures follow a lag, log, stationary, and finally death phase. [6] The lag phase is not well known in microbiology, but it is speculated that this phase consists of the microorganism adjusting to its environment by synthesizing proteins specific for the surrounding habitat. [6]
In biology and biological engineering, the change in numbers of microbes due to asexual reproduction and nutrient exhaustion is commonly illustrated by a semi-log plot. Time is usually the independent axis, with the logarithm of the number or mass of bacteria or other microbe as the dependent variable. This forms a plot with four distinct ...
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]