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ELISA. The enzyme-linked immunosorbent assay (ELISA) (/ ɪˈlaɪzə /, / ˌiːˈlaɪzə /) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. [1] The assay is a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using ...
Assay. An assay is an investigative (analytic) procedure in laboratory medicine, mining, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity. The measured entity is often called the analyte, the measurand, or the target ...
e. Enzymes (/ ˈɛnzaɪmz /) are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme converts the substrates into different molecules known as products.
The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [ 1 ] Besides detecting the proteins, this technique is also utilized to visualize, distinguish, and quantify the ...
In cellular biology, active is the movement of molecules or ions across a cell membrane from a region of lower concentration to a region of higher concentration —against the concentration gradient. Active transport requires cellular energy to achieve this movement. There are two types of active transport: primary active transport that uses ...
Chemical biology is a scientific discipline between the fields of chemistry and biology. The discipline involves the application of chemical techniques, analysis, and often small molecules produced through synthetic chemistry, to the study and manipulation of biological systems. [1] Although often confused with biochemistry, which studies the ...
In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation and radiation, or heat. [3]
Biosensor. A biosensor is an analytical device, used for the detection of a chemical substance, that combines a biological component with a physicochemical detector. [1][2][3][4] The sensitive biological element, e.g. tissue, microorganisms, organelles, cell receptors, enzymes, antibodies, nucleic acids, etc., is a biologically derived material ...