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William Thomas Astbury FRS (25 February 1898 – 4 June 1961) was an English physicist and molecular biologist who made pioneering X-ray diffraction studies of biological molecules. [1] His work on keratin provided the foundation for Linus Pauling 's discovery of the α-helix .
When Wilhelm Röntgen discovered X-rays in 1895 [1] physicists were uncertain of the nature of X-rays, but suspected that they were waves of electromagnetic radiation.The Maxwell theory of electromagnetic radiation was well accepted, and experiments by Charles Glover Barkla showed that X-rays exhibited phenomena associated with electromagnetic waves, including transverse polarization and ...
The first X-ray diffraction experiment was conducted in 1912 by Max von Laue, [7] while electron diffraction was first realized in 1927 in the Davisson–Germer experiment [8] and parallel work by George Paget Thomson and Alexander Reid. [9] These developed into the two main branches of crystallography, X-ray crystallography and electron ...
1905 - Charles Glover Barkla discovered the X-ray polarization effect. [45] 1908 - Bernhard Walter and Robert Wichard Pohl observed X-ray diffraction from a slit. [46] [47] 1912 - Max von Laue discovered diffraction patterns from crystals in an x-ray beam. [48]
The concept of Bragg diffraction applies equally to neutron diffraction [4] and approximately to electron diffraction. [5] In both cases the wavelengths are comparable with inter-atomic distances (~ 150 pm). Many other types of matter waves have also been shown to diffract, [6] [7] and also light from objects with a larger ordered structure ...
An X-ray diffraction pattern of a crystallized enzyme. The pattern of spots (reflections) and the relative strength of each spot (intensities) can be used to determine the structure of the enzyme. The relative intensities of the reflections provides information to determine the arrangement of molecules within the crystal in atomic detail.
The interference achieved by a diffraction grating both improves the spectral resolution over a prism and allows for the dispersed wavelengths to be quantified. Fraunhofer's establishment of a quantified wavelength scale paved the way for matching spectra observed in multiple laboratories, from multiple sources (flames and the sun) and with ...
A year later, X-ray diffraction was further applied to visualize the three-dimensional structure of an unstained human chromosome. [20] X-ray microscopy has thus shown its great ability to circumvent the diffractive limit of classic light microscopes; however, further enhancement of the resolution is limited by detector pixels, optical ...