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Food vacuoles (also called digestive vacuole [27]) are organelles found in certain protists, such as ciliates, ...
The contractile vacuole, as its name suggests, expels water out of the cell by contracting. The growth (water gathering) and contraction (water expulsion) of the contractile vacuole are periodical. One cycle takes several seconds, depending on the species and the osmolarity of the environment.
Food vacuoles are formed through phagocytosis and typically follow a particular path through the cell as their contents are digested and broken down by lysosomes so the substances the vacuole contains are then small enough to diffuse through the membrane of the food vacuole into the cell.
The vacuole is prominent and present at the center of the cell, surrounded by cytoplasm. Firm, small onions are best for microscopy. The epidermal layers are removed by cutting the onion and peeling them off (they are the membrane-like sheaths between each onion layer).
It is the hyaloplasm of light microscopy, a highly complex, polyphasic system in which all resolvable cytoplasmic elements are suspended, including the larger organelles such as the ribosomes, mitochondria, plant plastids, lipid droplets, and vacuoles.
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Plant cells have a large central vacuole in the center of the cell that is used for osmotic control and nutrient storage. Contractile vacuoles are found in certain protists, especially those in Phylum Ciliophora. These vacuoles take water from the cytoplasm and excrete it from the cell to avoid bursting due to osmotic pressure.