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  2. Agarose gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Agarose_gel_electrophoresis

    Agarose gel electrophoresis is most commonly done horizontally in a subaquaeous mode whereby the slab gel is completely submerged in buffer during electrophoresis. It is also possible, but less common, to perform the electrophoresis vertically, as well as horizontally with the gel raised on agarose legs using an appropriate apparatus. [ 27 ]

  3. Gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Gel_electrophoresis

    Gel electrophoresis of large DNA or RNA is usually done by agarose gel electrophoresis. See the " chain termination method " page for an example of a polyacrylamide DNA sequencing gel. Characterization through ligand interaction of nucleic acids or fragments may be performed by mobility shift affinity electrophoresis .

  4. Gel doc - Wikipedia

    en.wikipedia.org/wiki/Gel_doc

    An example Gel documentation system, showing the results of gel electrophoresis on a connected monitor.. A gel doc, also known as a gel documentation system, gel image system or gel imager, refers to equipment widely used in molecular biology laboratories for the imaging and documentation of nucleic acid and protein suspended within polyacrylamide or agarose gels.

  5. File:Agarose Gel Electrophoresis - Assembling the Rig and ...

    en.wikipedia.org/wiki/File:Agarose_Gel...

    You are free: to share – to copy, distribute and transmit the work; to remix – to adapt the work; Under the following conditions: attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made.

  6. Southern blot - Wikipedia

    en.wikipedia.org/wiki/Southern_blot

    Agarose gel Tray with a stack consisting top down of a weight, paper towels, membrane of nitrocellulose or nylon, gel, salt solution and a slab of glass. Southern blot membrane after hybridization and rinsing. Southern blot agarose gel under ultraviolet illumination. Southern blot autoradiogram.

  7. Gel electrophoresis of nucleic acids - Wikipedia

    en.wikipedia.org/wiki/Gel_electrophoresis_of...

    For a standard agarose gel electrophoresis, 0.7% gel concentration gives good separation or resolution of large 5–10kb DNA fragments, while 2% gel concentration gives good resolution for small 0.2–1kb fragments.

  8. Fluorescence imaging - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_imaging

    In PCR (agarose gel electrophoresis): SYBR Green is a very common dye that binds to DNA and is used to visualize DNA bands in an agarose gel. The dye absorbs blue light and fluoresces green for an imaging system to capture. Blotting (Western, Northern, and Southern): fluorochromes can bind to antibodies, RNA, and DNA to fluoresce and quantify data

  9. Electrophoretic mobility shift assay - Wikipedia

    en.wikipedia.org/wiki/Electrophoretic_mobility...

    A mobility shift assay is electrophoretic separation of a protein–DNA or protein–RNA mixture on a polyacrylamide or agarose gel for a short period (about 1.5-2 hr for a 15- to 20-cm gel). [4] The speed at which different molecules (and combinations thereof) move through the gel is determined by their size and charge, and to a lesser extent ...