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In autecological studies, the growth of bacteria (or other microorganisms, as protozoa, microalgae or yeasts) in batch culture can be modeled with four different phases: lag phase (A), log phase or exponential phase (B), stationary phase (C), and death phase (D). [3] During lag phase, bacteria adapt themselves to growth conditions. It is the ...
The first phase is the fast growth phase, since the bacterium is consuming (in the case of the above example) exclusively glucose, and is capable of rapid growth. The second phase is a lag phase while the genes used in lactose metabolism are expressed and observable cell growth stops.
When microorganisms from this culture are transferred into fresh media, nutrients trigger the growth of the microorganisms which will go through lag phase, a period of slow growth and adaptation to the new environment, followed by log phase, a period where the cells grow exponentially.
For protein expression and purification in bacteria it is recommended that protein induction and cell harvesting should be done at specific OD 600 (usually at the end of the log phase, OD 600 = 0.4). OD 600 is preferable to UV spectroscopy when measuring the growth over time of a cell population because at this wavelength, the cells will not be ...
This open system allows researchers to maintain the exponential growth phase of cells for use in physiological experiments. [ 1 ] A chemostat (from chem ical environment is stat ic) is a bioreactor to which fresh medium is continuously added, while culture liquid containing left over nutrients, metabolic end products and microorganisms is ...
The lag phase is not well known in microbiology, but it is speculated that this phase consists of the microorganism adjusting to its environment by synthesizing proteins specific for the surrounding habitat. [6] The log phase is the period where a culture experiences logarithmic growth until nutrients become scarce. The stationary phase is when ...
This page was last edited on 1 April 2013, at 17:59 (UTC).; Text is available under the Creative Commons Attribution-ShareAlike 4.0 License; additional terms may ...
Bacterial growth curve. Fermentation begins once the growth medium is inoculated with the organism of interest. Growth of the inoculum does not occur immediately. This is the period of adaptation, called the lag phase. [7]