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Textbook of Biochemistry is divided into the following chapters: [2] Introduction; Introduction to the concept of biochemistry, and a review of catalytic reactions and pH. Food-Stuffs, Their Derivatives and Related Substances. Ideas regarding carbohydrates, lipids, and proteins. The Chemistry of Digestion, the Circulation, and the Excreto.
Biochemistry – study of chemical processes ... electrophoresis – chromatography – mass spectrometry – x-ray diffraction – Southern blot – fractionation ...
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the stationary phase is fixed ...
Ion chromatography (or ion-exchange chromatography) is a form of chromatography that separates ions and ionizable polar molecules based on their affinity to the ion exchanger. [1] It works on almost any kind of charged molecule —including small inorganic anions, [ 2 ] large proteins , [ 3 ] small nucleotides , [ 4 ] and amino acids .
Size-exclusion chromatography; Sørensen formol titration; Spin column-based nucleic acid purification; Stable isotope labeling by amino acids in cell culture; Stains-all; Summation theorems (biochemistry) Surface plasmon resonance; Surface-enhanced laser desorption/ionization
The Rossmann fold is a tertiary fold found in proteins that bind nucleotides, such as enzyme cofactors FAD, NAD +, and NADP +.This fold is composed of alternating beta strands and alpha helical segments where the beta strands are hydrogen bonded to each other forming an extended beta sheet and the alpha helices surround both faces of the sheet to produce a three-layered sandwich.
Sealed chromatography cartridges or columns work similarly except the sample and buffer is pumped into and through the resin by an external device such as a liquid chromatographic (LC) system, also requiring collection and monitoring of several fractions. Even though this method is often semi-automated, using chromatography cartridges is ...
Physical biochemistry is a branch of biochemistry that deals with the theory, techniques, and methodology used to study the physical chemistry of biomolecules. [1] It also deals with the mathematical approaches for the analysis of biochemical reaction and the modelling of biological systems .