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Phage display cycle. 1) fusion proteins for a viral coat protein + the gene to be evolved (typically an antibody fragment) are expressed in bacteriophage. 2) the library of phage are washed over an immobilised target. 3) the remaining high-affinity binders are used to infect bacteria. 4) the genes encoding the high-affinity binders are isolated.
Biopanning is an affinity selection technique which selects for peptides that bind to a given target. [1] All peptide sequences obtained from biopanning using combinatorial peptide libraries have been stored in a special freely available database named BDB.
QFX is a proprietary variant of OFX used in Intuit's products. In Intuit products, OFX is used for Direct Connect and QFX for Web Connect. Direct Connect allows personal financial management software to connect directly to a bank OFX server, whereas in Web Connect, the user needs to log in and manually download a .qfx file and import it into Quicken.
The SQL Server Network Library is controlled through the use of a Client Network Utility, which is bundled with the SQL Server. Each Net-Lib supported network protocol has a separate driver (not to be confused with a device driver), and has support for a session layer in its protocol stack. There are two general types of Net-Lib: the primary ...
Assembled major coat protein, exploded view. The virion is a flexible filament (worm-like chain) about 6 nm in diameter and 900 nm long.Several thousand copies of a small (50 amino-acid residues) elongated alpha-helical major coat protein subunit (the product of gene 8, or p8) in an overlapping shingle-like array form a hollow cylinder enclosing the circular single-stranded DNA genome.
Phage typing is based on the specific binding of phages to antigens and receptors on the surface of bacteria and the resulting bacterial lysis or lack thereof. [4] The binding process is known as adsorption. [5] Once a phage adsorbs to the surface of a bacteria, it may undergo either the lytic cycle or the lysogenic cycle. [6]
The RNA-dependent RNA polymerase that replicates both the positive and negative RNA strands is a complex of four proteins: the catalytic beta subunit (replicase, ) is encoded by the phage, while the other three subunits are encoded by the bacterial genome: alpha subunit (ribosomal protein S1), gamma subunit (), and delta subunit ().
Plaque exhibiting bacterial lawn with clearings made by Artharobacter phage GantcherGoblin.. The Actinobacteriophage database, more commonly known as PhagesDB, is an interactive, comprehensive, database-backed website that collects and shares information related to the discovery, characterization and genomics of viruses that typically infect Actinobacterial hosts.