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Incubation period (also known as the latent period or latency period) is the time elapsed between exposure to a pathogenic organism, a chemical, or radiation, and when symptoms and signs are first apparent. [1]
Once the growth medium in the petri dish is inoculated with the desired bacteria, the plates are incubated at the optimal temperature for the growing of the selected bacteria (for example, usually at 37 degrees Celsius, or the human body temperature, for cultures from humans or animals, or lower for environmental cultures). After the desired ...
The incubator maintains optimal temperature, humidity and other conditions such as the CO 2 and oxygen content of the atmosphere inside. Incubators are essential for much experimental work in cell biology , microbiology and molecular biology and are used to culture both bacterial and eukaryotic cells.
The bacteria are spread by airborne droplets and the disease's incubation period is 7–10 days on average (range 6–20 days). [1] [2] Humans are the only known reservoir for B. pertussis. [3] The complete B. pertussis genome of 4,086,186 base pairs was published in 2003. [4]
Its incubation period lasts 30 minutes to eight hours, [41] with the illness itself lasting from 30 minutes to 3 days. [42] Preventive measures one can take to help prevent the spread of the disease include washing hands thoroughly with soap and water before preparing food.
The bacteria are mesophilic and grow best at temperatures between 35 and 37 °C. [1] H. influenzae was first described in 1893 [2] [3] by Richard Pfeiffer during an influenza pandemic [4] when he incorrectly identified it as the causative microbe, which is why the bacteria was given the name "influenzae".
S. marcescens is a motile organism and can grow in temperatures ranging 5–40 °C (41–104 °F) and in pH levels ranging from 5 to 9. It is differentiated from other Gram-negative bacteria by its ability to perform casein hydrolysis, which allows it to produce extracellular metalloproteinases which are believed to function in cell-to-extracellular matrix interactions.
The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample. From here, the plates are rotated to ensure the samples are uniformly mixing with the agar. Incubation of the plates is the next step and is carried out for about 3 days at 20 to 30 degrees Celsius.