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ChemBioChem is a peer-reviewed scientific journal covering chemical biology, synthetic biology, and bio-nanotechnology and published by Wiley-VCH on behalf of Chemistry Europe. The journal publishes communications, full papers, reviews, minireviews, highlights, concepts, book reviews, and conference reports.
18-Crown-6 can be synthesized by the Williamson ether synthesis using potassium ion as the template cation. Structure of nickel-aquo nitrate complex of the ligand derived from the templated trimerization of 2-aminobenzaldehyde. [5] The phosphorus analogue of an aza crown can be prepared by a template reaction. [6]
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The gene of interest with its flanking regions is amplified to introduce these SeSaM_fwd and SeSaM_rev sequences and to generate template for consecutive PCR steps. These generated so-called fwd template and rev templates are now amplified in a PCR reaction with a pre-defined mixture of phosphorothioate and standard nucleotides to ensure an ...
[6] [7] The cell extract-based type are susceptible to problems like quick degradation of components outside their host, as shown in a study by Kitaoka et al. where a cell-free translation system based on Escherichia coli (E. coli), of the cell extract-based type, had the mRNA template degrade very quickly and led to the halt of protein ...
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Nucleic acid NMR is the use of NMR spectroscopy to obtain information about the structure and dynamics of nucleic acid molecules, such as DNA or RNA.As of 2003, nearly half of all known RNA structures had been determined by NMR spectroscopy.
Enzyme promiscuity is the ability of an enzyme to catalyze an unexpected side reaction in addition to its main reaction. Although enzymes are remarkably specific catalysts, they can often perform side reactions in addition to their main, native catalytic activity.