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The Sørensen formol titration(SFT) invented by S. P. L. Sørensen in 1907 [1] is a titration of an amino acid with potassium hydroxide in the presence of formaldehyde. [2] It is used in the determination of protein content in samples. [3] Formol titration equation for amino acids in general
The NeuCode amino acid method is similar to SILAC but differs in that the labeling only utilizes heavy amino acids. The use of only heavy amino acids eliminates the need for 100% incorporation of amino acids needed for SILAC. The increased multiplexing capability of NeuCode amino acids is from the use of mass defects from extra neutrons in the ...
Coupled system consisting of three acids. The black curve shows a back-titration event. When a protein folds, the titratable amino acids in the protein are transferred from a solution-like environment to an environment determined by the 3-dimensional structure of the protein.
The Van Slyke determination is a chemical test for the determination of amino acids containing a primary amine group. It is named after the biochemist Donald Dexter Van Slyke (1883-1971). [1] One of Van Slyke's first professional achievements was the quantification of amino acids by the Van Slyke determination reaction. [2]
BCA protein assay in a 96 well plate. The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, [1] now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or ...
The entire experiment is done at room temperature. The Bradford protein assay can measure protein quantities as little as 1 to 20 μg. [14] It is an extremely sensitive technique. The dye reagent is a stable ready to use product prepared in phosphoric acid. It can remain at room temperature for up to 2 weeks before it starts to degrade.
Stable isotope labeling with amino acids in cell culture is a procedure that can be done in vivo. This procedure can be used in all cell culture laboratories and is a routinely used labeling technique. This metabolic labeling enables inhibition of a given protease in biological samples and analysis of ex vivo processing. [1]
2-, alpha-, or α-amino acids [21] ... Composite of titration curves of twenty proteinogenic amino acids grouped by side ... In the famous Urey-Miller experiment, ...