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A pair of ZFNs, each with three zinc fingers binding to target DNA, are shown introducing a double-strand break, at the FokI domain, depicted in yellow. Subsequently, the double strand break is shown as being repaired through either homology-directed repair or non-homologous end joining. [3]
Afterwards, the 3’ ssDNA invades the template DNA, and displaces a DNA strand to form a D-loop. DNA polymerase and other accessory factors follows by replacing the missing DNA via DNA synthesis. Ligase then attaches the DNA strand break, [10] resulting in the formation of 2 Holliday junctions. The recombined DNA strands then undergoes ...
In addition to histidine, a conserved arginine on the second beta strand of the zinc fingers makes contact with the phosphodiester oxygen on the DNA strand. [25] [26] [29] Also serine 75 on the third finger hydrogen bonds to the phosphate between base pairs 7 and 8, as the only backbone contact with the secondary strand of DNA. [25] [26] [29]
Two ligands from a knuckle and two more from the c terminus of a helix. Gag knuckle: Two ligands from a knuckle and two more from a short helix or loop. Treble clef: Two ligands from a knuckle and two more from the N-terminus of a helix. Zinc ribbon: Two ligands each from two knuckles. Zn 2 /Cys 6: Two ligands from the N terminus of a helix and ...
After strand invasion, the further sequence of events may follow either of two main pathways discussed below (see Models); the DSBR (double-strand break repair) pathway or the SDSA (synthesis-dependent strand annealing) pathway. Homologous recombination that occurs during DNA repair tends to result in non-crossover products, in effect restoring ...
The filament searches for the homologous chromosome, strand invasion occurs where the new chromosome forms a D-loop over the bottom sister chromatid, then the ends are annealed. This process can yield double Holliday junctions that when cut in a transversal pattern by endonucleases form 2 heteroduplex strand products.
In the C programming language, Duff's device is a way of manually implementing loop unrolling by interleaving two syntactic constructs of C: the do-while loop and a switch statement. Its discovery is credited to Tom Duff in November 1983, when Duff was working for Lucasfilm and used it to speed up a real-time animation program.
The DNA is cut 9 nucleotides downstream of the motif on the forward strand, and 13 nucleotides downstream of the motif on the reverse strand, [3] producing two sticky ends with 4-bp overhangs. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.