Search results
Results from the WOW.Com Content Network
The distribution constant (or partition ratio) (K D) is the equilibrium constant for the distribution of an analyte in two immiscible solvents. [1] [2] [3]In chromatography, for a particular solvent, it is equal to the ratio of its molar concentration in the stationary phase to its molar concentration in the mobile phase, also approximating the ratio of the solubility of the solvent in each phase.
[10]: 280–4 Hence, a single experiment can be used to measure the logarithms of the partition coefficient (log P) giving the distribution of molecules that are primarily neutral in charge, as well as the distribution coefficient (log D) of all forms of the molecule over a pH range, e.g., between 2 and 12.
Sodium dihydrogen phosphate Sodium hydrogen phosphate Trisodium phosphate. A sodium phosphate is a generic variety of salts of sodium (Na +) and phosphate (PO 3− 4). Phosphate also forms families or condensed anions including di-, tri-, tetra-, and polyphosphates. Most of these salts are known in both anhydrous (water-free) and hydrated forms ...
Disodium phosphate (DSP), or disodium hydrogen phosphate, or sodium phosphate dibasic, is an inorganic compound with the chemical formula Na 2 H P O 4. It is one of several sodium phosphates . The salt is known in anhydrous form as well as hydrates Na 2 HPO 4 · n H 2 O , where n is 2, 7, 8, and 12.
Monosodium phosphate (MSP), also known as monobasic sodium phosphate and sodium dihydrogen phosphate, is an inorganic compound with the chemical formula Na H 2 P O 4. It is a sodium salt of phosphoric acid. It consists of sodium cations (Na +) and dihydrogen phosphate anions (H 2 PO − 4). One of many sodium phosphates, it is a common ...
A modern self-contained HPLC Schematic representation of an HPLC unit (1) solvent reservoirs, (2) solvent degasser, (3) gradient valve, (4) mixing vessel for delivery of the mobile phase, (5) high-pressure pump, (6) switching valve in "inject position", (6') switching valve in "load position", (7) sample injection loop, (8) pre-column (guard column), (9) analytical column, (10) detector (i.e ...
Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [ 1 ] [ 2 ] [ 3 ] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the ...
In chromatography substances are separated by partition between a stationary phase and a mobile phase. The analyte is dissolved in the mobile phase, and passes over the stationary phase. Separation occurs because of differing affinities of the analytes for the stationary phase. A distribution constant, K d can be defined as